Automated High-Content Screening
Precision and Efficiency in Drug Discovery

Infrastructure

At Pivot Park Screening Centre, we offer state-of-the-art High-Content Screening (HCS) services designed to revolutionize your early drug discovery process. Our advanced HCS system, equipped with an automated plate loader, ensures seamless operation, significantly reducing costs and increasing efficiency.

Advanced High-Content Screening with Plate Loader Automation

Our HCS platform is equipped with a sophisticated plate loader and robotic automation, ensuring seamless and efficient operations. This automated system significantly reduces manual labor and operational costs, allowing you to focus your resources where they matter most.

High-quality Imaging for phenotypic analysis

Experience the highest quality imaging with our High Content Imager, the Thermo Scientific™ CellInsight™ CX7 LZR Pro, featuring the highest quality sCMOS camera offering 95% quantum efficiency. Our imaging capabilities provide exceptional detail and accuracy, whether you’re conducting cell painting assays or advanced 2D or 3D cell culture assays. This allows for more comprehensive data analysis and confident decision-making.

Comprehensive Assay Services for every need

At Pivot Park Screening Centre, we support every type of assay. From luminescence to complex 3D cell culture assays, we have the expertise and equipment to meet your needs. Our in-house capabilities extend to target validation, ensuring your research is thorough and reliable. Our integrated approach ensures a smooth transition from initial screening to detailed validation, all under one roof at Pivot Park Screening Centre.

Your Partner in Phenotypic Drug Discovery

We offer both ultra-high throughput screening (uHTS) and automated high-content screening (HCS) to support your (phenotypic) drug discovery efforts. Our experience in automation and assay development will ensure your projects’ success.

Why choose Pivot Park Screening Centre?

State-of-the-art Technology: Leverage the best camera and imaging technology for detailed and accurate results.
High Level of Automation: Benefit from our automated systems to save time, reduce costs and ensure efficiency and reliability
Expert Support: Our specialists in assay development are here to help you every step of the way.
Comprehensive Services: From primary screening to hit validation and target validation, we offer a complete range of services.

Take the next step in you research journey

Partner with Pivot Park Screening Centre for your drug discovery needs. Our advanced HCS solutions, combined with our expert team and services, will further your research.

Ready to get started?

What is High-Content Screening?

High-Content Screening involves the automated acquisition and analysis of images from biological samples, such as cells or tissues. Unlike traditional methods, HCS utilizes sophisticated imaging systems capable of capturing multiple parameters simultaneously, providing a comprehensive description of cellular morphology.

How does High-Content Screening work?

High-Content Screening systems typically consist of automated microscopes equipped with high-resolution cameras, robotic sample handling systems, and powerful image analysis software. These systems can capture images of thousands of cells in a single experiment, enabling high-throughput screening and analysis.

Key Features of High-Content Screening:

Multiplexing: HCS enables the simultaneous detection of multiple biomolecules within a single sample, offering insights into complex cellular interactions.
High Throughput: Automating image acquisition and analysis accelerates research, facilitating screening of large compound libraries or genetic screens.
Quantitative Analysis: HCS generates precise quantitative data, allowing researchers to measure parameters such as cell morphology, protein expression levels, and subcellular localization.

Applications of High-Content Screening

Drug Discovery and Development: Widely used in drug discovery pipelines for screening compound libraries and studying drug mechanisms of action.
Cellular Phenotypic Profiling: HCS helps characterize cellular phenotypes in response to genetic or environmental changes, thereby identifying novel therapeutic targets.

High-Content Screening (HCS) provides a comprehensive approach to studying cellular and molecular processes, offering researchers a wealth of data to uncover new insights and advance scientific discovery. With its ability to capture complex biological phenomena in high throughput, HCS continues to drive innovation across various fields of research. Partner with Pivot Park Screening Centre to leverage HCS in your research endeavors.

The High-Content Screening System at Pivot Park screening Centre

At Pivot Park Screening Centre we make use of an Ultrasensitive epi-fluorescent imaging and analysis screening platform with precision automated stage, SBS compatible plate insert, advanced software autofocus, on-board laser autofocus (730nm) and laser-scanning barcode reader. Includes 16-bit scientific CMOS Photometrics camera with 95% quantum efficiency and low 1.0 e- read noise.

We have the following high-quality Olympus X-line objectives in place:

Magnification	NA	WD
4x	0.16	13 mm
10x	0.4	3.1 mm
10x	0.45	6.6-7.8 mm
10x	0.6	2.7-4.0 mm
10x	0.7	0.8-1.8 mm
20x	0.8	0.6 mm
40x	0.95	0.18 mm
60x	0.9	0.2 mm

Includes seven color laser excitation capability: 405nm, 450nm, 488nm, 561nm, 594nm, 647nm, and 785nm.

The system is configured with multimode distinct channel imaging with enabled wide field, dual patterned spinning disk confocal, and five color transmitted light capabilities.

Interested? Get in touch and we’ll help you accelerate your drug discovery.

Ultra High Throughput Screening infrastructure in a nutshell. Watch our video

Are you a startup?

Read all about the 5 reasons to outsource a screen at PPSC

Watch our webinars about HCS

Working with the High Content Screening System at Pivot Park Screening Centre

News

G2Solutions: funding for innovation in single-cell genomics.

The G2Solutions project, led by Prof. Dr. Ir. Floris Foijer from the UMCG – ERIBA Research Institute, has received funding

21 October 2024

Events

Discovery US 2024 in Boston, November 7-8

Join us at Discovery US and network with us and over 250 industry leaders. We will be there to discuss the latest advancements in ultra-High Throughput Screening, Mass Spectrometry, target identification, validation and HIT optimization.

9 September 2024