Tailored biochemical and cellular
assay development solutions

An enthusiastic team of experienced scientists provide you with industry-standard solutions for tailored cellular, biochemical and biophysical assay development, optimization and miniaturization to support your lead discovery projects.

The right assay for your target

Our scientists have longstanding and proven experience on the most challenging targets. At Pivot Park Screening Centre, we believe each project has unique aspects, and we collaborate with you to deliver tailored and high-quality assays. Pivot Park Screening Centre achieves this by detailed scientific assessment of your project requirements, and tailors a proposal ranging from a reliable screening cascade to a stand-alone assay to address your particular needs. No matter how challenging your target is, our scientists will ideate out-of-the-box solutions and propose the best fitting approach to develop the right assay suitable for target evaluation, ultra High Throughput Screening (uHTS)hit triaging and hit-to-lead biological profiling. We have broad experience in a variety of phenotypes and target classes ranging from kinases, GPCRs and ion channels to nuclear receptors, proteases and protein interactions with other proteins or nucleic acids in many therapeutic areas.

Label-based and label-free biochemical, cellular and biophysical assay development

We provide a broad range of label-based and label-free biochemical, cellular and biophysical assays that are optimised to deliver high-quality data. Our scientists have ample experience in a variety of readout technologies including the most conventional label-based biochemical and cellular readouts such as Fluorescence, FRET/HTRF/TR-FRET, FP, Luminescence, absorption, high-content, phenotypic, as well as label-free MALDI-TOF mass spectrometry biochemical, affinity-based and cellular assays.

We offer a proprietary chemical biology framework to identify assay liabilities towards various types of readout interferences along with undesired non-drug-like modes-of-action, and tailor primary assay conditions and triaging assay cascade in a data-driven manner to guarantee delivery of high-quality and validated hits.

A customised solution

Pivot Park Screening Centre actively pursues customised solutions for profiling novel classes of small molecule modalities such as PROTACs and molecular glues. We also continuously work towards integrating novel disease models based on patient-derived or Crispr/Cas edited induced Pluripotent Stem Cells (iPSC) models such as cardiomyocytes and neuronal cells into our screening capabilities.

Assay development - Working at Pivot Park Screening Centre

Reduction of assay interference with our robustness set

Besides the quality of the library and the primary assay, the success of any uHTS campaign is largely dependent on the post-screen hit-triaging phase, particularly because of undesired compound-assay interference leading to false-positive data. The undesired false-positive effects of these compounds disturb the hit selection process, thus requiring orthogonal assay readouts and adjustments in the primary assay conditions. 

For organizations running many uHTS assays with the same compound library, such interfering molecules become recognized as “frequent hitters.” The main causes for such interference are aggregation, metal ion chelation, redox activity, auto-fluorescence, absorbance, luciferase inhibition, and chemical reactivity. To dissect such liabilities early in the assay development phase, we make use of the “robustness set” compound collection. The robustness set comprises of selected molecules from the literature with well-defined assay interfering properties and so-called clean compounds for which we do not expect assay interference. These are supplemented with many DMSO controls, all in a ready-to-employ single 1536-well plate. The application of the robustness set not only provides a framework to identify assay liabilities and tailor uHTS assay conditions such that minimal interference occurs but also helps to design suitable follow-up assay cascades for successful hit triaging.

Whether you are looking for high-quality assay development based on established techniques, or for more innovative novel assays, the passionate Pivot Park Screening Centre team will strive to deliver what your drug discovery project needs.

Disease areas

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Target classes

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Read-out Technologies

Fluorescent Intensity
Absorption Based Measurement
Fluorescent Polarisation
Time Resolved Fluorescence (FRET, TR FRET, HTRF)
Calcium Kinetics
Calcium Permeability
Potassium Kinetics
High Content Screening
Mass Spectrometry

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Thank you for you interest!

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